熒光手電筒用于觀察茜素紅染色的海馬骨架

熒光手電筒用于觀察茜素紅染色的海馬骨架

用于骨架的染料是茜素紅 S。它可以在白光下觀察，但在適當波長的激發(fā)下它會發(fā)出美麗的紅色。該圖像是在配備體視顯微鏡熒光適配器的體視顯微鏡上拍攝的，使用綠色 (GR) 激發(fā)光源和匹配的紅色屏障濾光片。

染紅的骨骼會在熒光手電筒照射下發(fā)出熒光

Fluorescence of Cleared and Stained Specimens

We received this beautifully detailed fluorescence image of a seahorse skeleton from Kevin Conway at Texas A&M University. The specimen, a dwarf seahorse (Hippocampus zosterae), was prepared using a technique called ‘clearing and staining’, a method that produces an intact specimen with cartilage stained blue and bones stained red. It is a great way visualize the entire structure of an organism.

The stain used for the skeleton is Alizarin Red S. It can be viewed under white light, but under appropriate excitation it glows a beautiful red. The image was made on a stereo microscope outfitted with the Stereo Microscope Fluorescence Adapter, using the Green (GR) excitation source and matching red barrier filter.

染紅的骨骼會在熒光手電筒照射下發(fā)出熒光

Morphological variation in support of the relationships among the Centropomoidei. Images of cleared and stained specimens fluorescing under royal blue light. Presence of rostral extension of external process on maxilla (character 361)—(A) Centropomus undecimalis (FMNH 77806), arrow, lateral view of right maxilla, scale bar = 5 mm; (B) Centropomus undecimalis (FMNH 77806), arrow, dorsal view of right maxilla, scale bar = 5 mm; (C) Sphyraena barracuda (FMNH 74209), arrow, lateral view of right maxilla, scale bar = 5 mm; (D) Sphyraena barracuda (FMNH 74209), arrow, dorsal view of right maxilla, scale bar = 5 mm. Enlargement of the second abdominal neural spine (character 1621)—(E) Lates calcarifer (AMNH 37839), arrow, right lateral view, scale bar = 1 mm; (F) Centropomus undecimalis (FMNH 77806), arrow, right lateral view, scale bar = 2.5 mm; (G) Lactarius lactarius (KUI 41405), arrow, right lateral view, scale bar = 5 mm. Possession of elongated, fang-like teeth in the oral jaws (character 261) and oral teeth that are ankylosed to the premaxilla and dentary (character 271)—(H) Sphyraena idiastes (SIO 15–182), lateral view of right premaxilla, scale bar = 1 mm; (I) Lactarius lactarius (KUI 41405), lateral view of right premaxilla, scale bar = 1 mm; (J) Lactarius lactarius (KUI 41405), lateral view of right dentary, scale bar = 1 mm.

上海峰志儀器有限公司銷售的激發(fā)光源，能夠激發(fā)茜素紅等多種熒光素發(fā)出熒光，詳情請電話或.咨詢網頁底部.

LUYOR-3415RG便攜式熒光激發(fā)光源

一直以來，脊椎動物研究人員都靠所謂的清理和染色的方法（剝離標本的軟組織，用紅色染料染色），拍攝詳細的圖片，進行解剖檢查，分析物種之間的關系。但由于失去了韌帶和肌肉組織，骨骼松弛，很難支撐，科學家無法從特定角度進行拍攝。脊椎動物研究人員利用這些圖像來探索動物的進化過程，并辨別它們與其他物種的共同特征。

如果沒有組織，像白眼蟒這樣的動物，很多骨骼會柔弱無骨，無法定型。但明膠能把它們固定住，拍完照片后再清洗掉。

在LUYOR-3280或LUYOR-3415熒光手電筒照射下，甘油明膠基質支撐的紅色染料染過的海馬骨骼發(fā)著光。這種攝影技術可以幫助科學家用一種新方法檢查骨骼。